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Pdonr221 P4R-P3R Sequence And Map

Di: Jacob

Use text editor or plasmid mapping software to view sequence. Plasmid Resuspension Protocol: 1.

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mapPercentage of construct sequence that has been empirically verified through direct sequence data.Sequence Analyzer.In this experiment, the protospacer sequence was at an ideal position as it extended across the end of the coding sequence to the beginning of the 3′ untranslated region and included the DNA encoding the stop codon (Figure (Figure5b).

pDONR 221 P1-P4 BioVector NTCC质粒载体菌种细胞基因保藏中心 - Biovector质粒载体菌种细胞蛋白抗体基因 ...

unique vector overhangs allow for directional ligation of custom oligonucleotides containing the protospacer sequence.Schlagwörter:Pdonr221 VectorAddgene

Gateway™ pDONR™221 Vector

Browse a digital-only collection of vector backbone informationSchlagwörter:Pdonr221 VectorPdonr221 SizeVector Database View Map in NovoBuilder . Gain unparalleled visibility of .rrnB T2 transcription termination sequence (c): rrnB T1 transcription termination sequence (c): M13 Forward (-20) priming site: attP1: ccdB gene (c): Chloramphenicol resistance . Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, files and maps Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, files and maps

pDEST17 Sequence and Map

Addgene: pDONR221-dyrk1ab

Analyze a DNA sequence to see restriction sites and map.Provide pDONR221 P5-P4 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

pDONR201 Sequence and Map

Vector Database.Provide pDONR221 P4r-P3r vector/plasmid map, full length sequence, antibiotic resistance, size and other informationSchlagwörter:Pdonr221 VectorPdonr221 Size

pDONR 221 P4r-P3r

Description: Gateway®, donor (entry), recombinational cloning clone, bacterial . Set the Destination Vector Select the Destination Vector tab and choose the destination vector from the list of Standard Destination Vectors. Not for use in diagnostic . Stable Unspecified Constitutive Unspecified Viral/Non-Viral Unspecified. Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, files and maps

Simulate Gateway® Cloning with One Insert

For Research Use Only. pDONR™221 P4r-P3r 4717 bp M13 Forward M13 attP4r ccdB CmR attP3r Reverse Comments for pDONR™221 P4r . Reverse: AttP2.comInvitrogen™ Gateway™ pDONR™221 Vector – Fisher Scifishersci. Explore the Scientific R&D Platform.Schlagwörter:Pdonr221 VectorDonor Vector

Vector Details: pDONR221

The pDONR™221 vector has a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use. Image: Illustrated plasmid map in PNG format.Schlagwörter:Pdonr221 VectorDonor VectorGateway Cloning Lr Reaction

pET-DEST42 Sequence and Map

Addgene: pDONR221-kcnk2a

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, files and maps

Simulate Gateway® Cloning with Multiple Inserts

An open DNA file will automatically be preselected as the insert for Gateway cloning.Commonly used Gateway® sequences including Donor Vectors, Entry Vectors, and Destination Vectors .

pDONR221-P1P4载体图谱质粒图谱、序列、价格、抗性、测序引物、大小等基本信息_生物风载体

Gateway® donor vector with attP1 and attP2 sites and a kanamycin resistance marker.comEmpfohlen auf der Grundlage der beliebten • Feedback

pDONR221 P4r-P3r vector map and sequence

pDONR™221 P4r-P3r 4717 bp M13 Forward M13 attP4r ccdB CmR attP3r Reverse Comments for pDONR™221 P4r-P3r 4717 base pairs rrnB T2 transcription termination sequence: bases 268-295 (c) rrnB T1 transcription termination sequence: bases 427-470 (c) M13 Forward (-20) priming site: . GenBank File: Plasmid sequence and annotations.Provide pDONR221 P3-P2 vector/plasmid map, full length sequence, antibiotic resistance, size and other informationSchlagwörter:Pdonr221 VectorPdonr221 Size

pDONRTM A P4-P1R

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map

pDONR221

Plasmid pDONR221_EGFP from Dr. Define the Insert Fragment. Sequence Author: .Provide pDONR221 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pDONR™221 P1-P4 4774 bp M13 Forward M13 attP1 ccdB CmR attP4 Reverse Comments for pDONR™221 P1-P4 4774 base pairs rrnB T2 transcription termination sequence: bases 268-295 (c) rrnB T1 transcription termination sequence: bases 427-470 (c) M13 Forward (-20) priming site: . Commonly used Gateway® sequences including Donor Vectors, Entry Vectors, and Destination Vectors.2665 3041 3471 3828 4597 4610 4731 BstUI 13 150 152 233 498 1246 1794 1841 3188 3238 3583 4062 4132 4713 BstXI 1 1306 BstYI 4 1833 3830 4104

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sequence

pDONR 221

In the above example, the vector pDONR221 P4r-P3r is suggested as the donor vector to create an Entry clone for part 2. 14:2128-2135, 2004. Not for use in diagnostic procedures.The sequences of the attP1 and attP2 sites in pDONR223 are identical to those in the original pDONR201 Entry vector.Schlagwörter:Donor VectorSnapGene

Simulate Gateway® Cloning with Multiple Inserts

(c) Plasmid maps of the destination vectors pMH-Cas9-gate, pMK-Cas9-gate, and pZeo-Cas9-gate for hygromycin, G418, .

pDONR221 Sequence and Map

Comments for pDONRTMP4-P1R 4777 nucleotides.Simulate an LR reaction between the pDONR221:insert plasmid and the destination vector pDEST17. In this lesson we will fuse a coding sequence (CDS) in-frame with the N-terminal HIS-tag encoded by the pDest17 expression vector. Requires ccdB resistant strain of bacteria.Provide pDONR221 P1-P5r vector/plasmid map, full length sequence, antibiotic resistance, size and other information Epub 2011 Feb 22.Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure; Download SnapGene .These PCR products were subsequently cloned into pDONR221-P4rP3r (Invitrogen) . Sequencing Primer: Forward: U19.Use text editor or plasmid mapping software to view sequence.Vector Name: pDONR221. (Empty Backbone) ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning Depositing Lab. Gain unparalleled visibility of your plasmids, DNA .

Addgene: pDONR221-ZNF534

Schlagwörter:Pdonr221 VectorPdonr221 Size

Addgene: pBSDONR P4r-P2

Centrifuge at 5,000×g for 5 min. Reference: Rual et al, Genome Res. This plasmid is available through Addgene.

pDONR221 vector map and sequence

Sequence Copy sequence Sequence.For Research Use Only.pDONR207 vector Vector Map.To make this vector, sequences from the GateWay cassettes of pDONR221 P4r-P3r and pDONR221 P3-P2 were subcloned into pBlueScript. An open portfolio of interoperable, industry leading products. Browse a digital-only collection of vector backbone informationSchlagwörter:AddgeneVector Database David Root’s lab contains the insert EGFP and is published in Nat Methods.Switch to each Donor Vector: panel in turn, and define the source sequence, and the region of the source sequence, for each fragment.Provide pDONR221_SLC22A31 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

Addgene: pDONR221

pDONR™221 P1-P4 4774 bp M13 Forward M13 attP1 ccdB CmR attP4 Reverse Comments for pDONR™221 P1-P4 .Provide pDONR221 P3-P2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

pENTR4 Sequence and Map

Gu et al Plant Physiol. In this instance, the protospacer sequence was not present on the DNA donor template. The Dotmatics digital science . Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, files and maps

pDEST14 Sequence and Map

( How to cite ) Sequence Information. SnapGene Viewer. In the above example, the vector .Provide pDONR221 P1-P4 vector/plasmid map, full length sequence, antibiotic resistance, size and other information

Efficient and modular CRISPR‐Cas9 vector system for

Gain unparalleled visibility of your plasmids, DNA and protein sequences; Annotate features on your plasmids using the curated feature database; Store, search, and share your sequences, files and maps

pDONR201 Sequence and Map

pDONR207 5585 bp 600 1200 1800 2400 3000 3600 4200 4800 5400 rrnB T2 terminator rrnB T1 terminator attP1 ccdB CmR cat promoter attP2 GmR Pc promoter ori. Perfect Match Genes [?] 100% full-length nucleotide and amino acid (for .

Addgene: pDONR221-ZNF883

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.Gateway Cloning Protocols | Thermo Fisher Scientific – USthermofisher. The alternative pDONR™221 vector seems to be preferred. The purpose of this was to create a . Carefully open the tube and add 20 μl of sterile water .提供 pDONR221 P4r-P3r 载体/质粒图谱，全长序列，抗性，大小和元件信息。 2011 Apr;155(4):1827-38. rrnB T2 transcription termination sequence: bases 268-295 (c) rrnB T1 transcription termination sequence: bases 427 .