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Phire Hot Start Ii Dna Polymerase

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Diese Eigenschaften . Includes Label or Dye. Thermo Scientific™ Phire™ Heißstart II DNA-Polymerase DNA-Polymerase ist das Produkt der Wahl für eine einheitlich stabile Amplifikation bei minimalem Optimierungsaufwand . Phire Hot Start II DNA-Polymerase ist ein neuartiges PCR-Enzym für Routineanwendungen und PCR-Anwendungen mit hohem Durchsatz. These features are achieved through advanced protein engineering of the polymerase.Phire Hot Start II DNA Polymerase (Cat.Thermo Scientific Phire Hot Start II DNA Polymerase is fused with a dsDNA-binding domain that allows short extension times (10–15 sec/kb) and helps improve yields compared to a standard hot-start Taq DNA . Die Phire Hot Start II DNA-Polymerase ist deutlich schneller, extrem zuverlässig und kann auch lange DNA . The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. For Use With (Application) Genotyping, Hot-start PCR. 该技术可提高 PCR 反应的特异性，同时无需额外的酶初始激活时间。 In addition, its hot-start technology with Affibody molecules allows complete activation of the enzyme in “zero-time” at standard . Diese Technologie erhöht die Spezifität der PCR-Reaktion, ohne dass zusätzliche Zeit für die anfängliche Aktivierung des Enzyms erforderlich ist. Water, nuclease-free to 50 µL to µL — 5X Phire™ Buffer1 10 µL µL 1X 10 mM dNTP mix 1 µL µL 0. For low complexity DNA (e. 1,000 reactions.

Phire Tissue Direct PCR Master Mix

A special DNA-binding domain linked to . For optimal results start by accurately calculating your .其中掺入了一段独特的双链 DNA 结合域，与 Taq DNA 聚合 . Phire Hot Start II DNA Polymerase also exhibits extremely high resistance to many PCR inhibitors found in plants.

Thermo Scientific Phire Green Hot Start II DNA Polymerase:PCR Equipment ...

Die Phire Hot Start II DNA-Polymerase ist deutlich schneller, extrem zuverlässig und kann auch lange DNA-Fragmente mit hohen Ausbeuten amplifizieren. Die Heißstart-Modifikation der Phire Hot-Start II DNA-Polymerase beruht auf der Affibody-Inaktivierungstechnologie. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), . Assistance technique Assistance client.No DNA purification is required prior to PCR. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols and others.5 kb Format: Separate components PCR setup Component 50-µL rxn Custom Final conc.Phire Hot Start II DNA Polymerase is a novel DNA polymerase designed for use in all routine and high throughput PCR applications.

Phire® Hot Start II DNA polymerase

Die einzigartige, doppelsträngige DNA-Bindungsdomäne ermöglicht kurze Extensionszeiten (10 bis 15 s/kb), verbessert die Ausbeute und erhöht die Wiedergabetreue um das 2-Fache im Vergleich zur Taq DNA-Polymerase.Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. SuperMix or Master .Die Hot-Start-Modifikation der Phire Hot Start II DNA-Polymerase basiert auf derselben Affibody-Inaktivierungstechnologie, die von der Phusion Hot Start II DNA-Polymerase verwendet wird. Die einzigartige, doppelsträngige DNA-Bindungsdomäne ermöglicht kurze Extensionszeiten .

Phire Hot Start II DNA Polymerase

It does not possess the 5´→3´ exonuclease activity needed for hydrolysis experiments.

Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific™ Phire Hot Start II DNA-Polymerase

Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold . Direct PCR Master Mix. Phire Hot Star t II DN . Use 10–15 s/kb for extension.Finnzymes’ Phire™ Hot Start DNA Polymerase is a novel PCR enzyme. Applications associées: PCR.Phire Hot Start II DNA Polymerase concentration is optimized to give good results in most reactions.

Guidelines for using Phire Hot Start DNA Polymerase

Taille de l’unité .

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase | Fisher Scientific

Ajouter à la liste Ajouter au panier. For high complexity genomic DNA, the amount of DNA template should be 25–250 ng per 50 μL reaction volume. Prix (EUR) Prix .40 EUR gültig bis . It does not possess the 5’ →3’ exonuclease activity needed for hy drolysis experiments. Genotyping; Transgene detection; The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.

Phire Hot Start II DNA-Polymerase

2 DNA-binding domain linked to the Phire DNA Polymerase

Wet or Dry Ice. Prix (EUR) Prix 640,00.The Master Mix employs Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA-binding domain that enhances the processivity of the polymerase.Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2 . Sie übertrifft jede auf Taq beruhende und auf dem Markt erhältliche Hot-Start-Polymerase. A special DNA .Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.Die Thermo Scientific Phire Hot-Start II DNA-Polymerase ist ein verbessertes PCR-Enzym für Routineanwendungen und PCR-Anwendungen mit hohem Durchsatz.Thermo Scientific™ Phire™ Hot Start II DNA Polymerase is a novel DNA polymerase designed for use in all routine and high throughput PCR applications.Overview Phire Hot Start DNA Polymerase is provided with 5x Phire Reaction Buffer. Only template and primers need to be added by the user.Thermo Scientific™ Lyo-ready Phire™ Hot Start II DNA Polymerase Enzyme characteristics Hot-start: Affibody™ technology Length: Up to 7. Sie übertrifft .Phire Hot Star t II DN A Polymerase generates blunt ends in the amplication products.Phire Hot Start II DNA Polymerase generates blunt ends in the amplification products. Référence: F122L.

Phire Hot Start II DNA Polymerase | Thermo Fisher Scientific - UK

Phusion™ Hot Start II DNA Polymerase (2 U/μL)

Reaction Format. The buffer contains 1.Phire Hot Start II DNA Polymerase. Diese Eigenschaften werden durch das fortschrittliche Protein-Engineering der Polymerase erzielt.

Thermo Scientific Phusion Hot Start II DNA Polymerase & dNTP Mix (10 mM ...

Marke: DreamTaq EP1703 626.Thermo Scientific Phire Hot Start II DNA Polymerase is fused with a dsDNA-binding domain that allows short extension times (10–15 sec/kb) and helps improve yields compared to a standard hot-start Taq DNA polymerase.Reagenzien und Kits für die Hot-Start-PCR Thermo Scientific™ Phire™ Heißstart II DNA-Polymerase 15619374 . Guidelines The annealing rules ar e different from many common DN A polymerases (such as Taq DN A polymerases). The polymerase incorporates a specific DNA-binding domain which shortens extension times, improves .Phire 热启动 II DNA 聚合酶的热启动修饰基于 Affibody 灭活技术。

Thermo Scientific Phire Hot Start II DNA Polymerase

Phire 热启动 II DNA 聚合酶具有强劲的性能和速度，它能够成功扩增长 DNA 片段，并且产量很高。 It outperforms every Taq -based hot start polymerase on the market. Taille de l’unité. plasmid, lambda or BAC DNA) it is recommended to use 2. Disponibilité-Quantité. Phire Green Hot Start II PCR Master Mix is a convenient 2X mix optimized reaction buffer including MgCl 2. The Phire Plant Direct PCR Master Mix contains reagents for . Votre prix Se connecter.Phire Hot Start II DNA Polymerase enables short extension times, improved PCR yields, and longer amplification compared to a standard hot-start Taq DNA polymerase.

Polymerase) Colony PCR (Protocol for Thermo Scientific™ Phire™ Hot Start II

Phire Hot Start II DNA Polymerase is a novel PCR enzyme for routine and high throughput PCR applications. • 快速热启动 —不需重新激活 . Reaction Speed.这些特点是通过聚合酶的高级蛋白质工程来实现的。 Built-In Hot Start.5 pg–25 ng per 50 μL reaction volume. F122L) provides high yields of specific product with short extension time (20 s/kb). Shipping Condition.